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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has long been noticed in several tumors, which leads to chromosome instability, and therefore may possibly contribute to tumorigenesis. This protein was discovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital role in marketing cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two further splice variants were described but thorough nucleotide sequence information will not be but available.

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Blocking peptides are peptides that bind particularly on the target antibody and block antibody binding. These peptide normally contains the epitope acknowledged because of the antibody.

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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible results are...

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This information outlines even further probable optimization of mobile and immunoassay parameters to be certain the very best success are...

Antibodies sure to the blocking peptide now not bind to your epitope to the focus on protein. By evaluating the staining through the blocked antibody compared to the antibody on your own, one can see which staining is specific.

Concentrate on Information and facts Cyclin E belongs to your hugely conserved cyclin household, whose associates are characterized by a spectacular periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead for the temporal coordination of each mitotic party. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This more info protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress through S period.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by each antibodies...

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